Detection of Human Papillomavirus Dna Among Women in Itesiwaju Local Government Area of Oyo State
DOI:
https://doi.org/10.51699/ijhsms.v2i9.2449Keywords:
Human Papillomavirus, DNA, Women.Abstract
In most developing countries, cervical cancer is one of the most prevalent diseases, and genital Human Papillomavirus (HPV) infection is a recognized cause of this cancer. Continuously, high-risk HPV infection, particularly strains 16 and 18, has been associated to cervical cancer. Currently available in Nigeria are the HPV vaccines Cervarix and Gardasil, which respectively target two (16 and 18) and four (6, 11, 16 and 18) strains. In Itesiwaju, a rural Local Government in Oyo State, women were enrolled in this study to look for Human Papillomavirus DNA. With residents from many ethnic groups, the Community is situated on a large piece of ground. Farming and mining are the two main occupations of the locals.
All the samples tested were collected from consenting women, some who are from farming communities and mining sites, others from other occupations and visit the public health facilities all around the Local Government. Information was collected from each participant using a questionnaire that captured demographic and sexual history. Genomic DNA was extracted from samples using commercial extraction reagents. The presence of HPV was detected by PCR using a primer (PGMY09/11) targeting E6/E7 genes. The PCR products were subjected to gel electrophoresis and visualized under a UV light.
A total of the 126 samples were tested and 14 of them were positive with HPV DNA giving an overall HPV prevalence rate of 11.1% in Itesiwaju. The largest proportion of participants (43.2%) fell within the 26-35 years age group, followed by the 36-45 years age group (30.7%). The 18-25 years age group accounted for 26.1% of the respondents.
The age range of 26 to 35 had the highest number of positive cases, which indicates a higher rate of sexual activity, according to subgroup analysis. These findings highlight the need for focused measures to address HPV infection in Itesiwaju LGA, such as awareness raising campaigns, vaccination programs, and routine screening. To comprehend the dynamics of HPV transmission and create effective preventative tactics that may be unique to the study region, more investigation and joint efforts are advised.
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