Detection of Concentration of CagA Protein of H Pylori in Both Dental Caries Patients and Non -Caries Patients by ELISA
DOI:
https://doi.org/10.51699/ijhsms.v2i8.2388Keywords:
CagA protein, H pylori, ELISA, dental cariesAbstract
Introduction: CagA protein, is a marker for the cag pathogenicity island which plays an important role in the etiology of H. pylori‑induced gastric pathologies. H pylori are detected in dental plaque, saliva and dental pulp. Saliva sample of 80 subjects with dental caries and free of caries is collected to detect the CagA level by ELISA technique. The results revealed no significant difference between two groups.
Conclusion: H-pylori are present in both groups which confirms that these bacteria alone cannot cause caries but it may cause later gastric ulcer.
Downloads
References
Moghaddam A, Mansouri Sh, Neshani A, Firoozeh F, Matinpur A, Khaledi A, Ghazalibina A. (2020). Construction, Cloning, and Expression of CagA Recombinant Protein of Helicobacter pylori. Avicenna J Med Biotech 2020; 12(2): 135-138
Neshani A, Zare H, Akbari Eidgahi MR, Khaledi A, Ghazvini K. Epinecidin-1, a highly potent marine anti-microbial peptide with anticancer and immunomodul- atory activities. BMC Pharmacol Toxicol 2019;20(1):33.
Abadi AH, Khaledi A, Bahador A, Mahdavi M, Esmaeili D. Expression, purification and characterization of the mixed total-OMP-CagA from brucella abortus and Heli-cobacter pylori as vaccine candidate. J Pure Appl Micro-biol 2016;10(3):2461-2467.
Han FC, Yan XJ, Su CZ. Expression of the CagA gene of H. pylori and application of its product. World J Gast-roenterol 2000;6(1):122-124.
El Batawi HY, Venkatachalam T, Francis A, Abujabal R, Shehadat SA. (2020)Dental Caries-A Hiding Niche for Helicobacter Pylori in Children. J Clin Pediatr Dent. 44(2):90–4.
Bıcak DA, Akyuz S, Kıratlı B, Usta M, Urganci N, Alev B, Yarat A, Sahin F. (2017)The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints. BMC Oral Health.;17(1):1–12
Abadi ATB, Mobarez AM, Teymournejad O, Karbalaei M. (2014)Concomitant colonization of Helicobacter pylori in dental plaque and gastric biopsy. J Pathogens:1.
Mehdipour A, Chaboki P, Rasouli R, Aghaali M, Sharifinejad N, Shams S (2023) Comparing the prevalence of Helicobacter pylori and virulence factors cagA, vacA, and dupA in supra‑gingival dental plaques of children 2 with and without dental caries: a case–control study BMC Oral Health 22:170 P 2 - 7
Al Asqah M, Al Hamoudi N, Anil S, Al-hamoudi WK.(2009) Is the presence of Helicobacter pylori in the dental plaque of patients with chronic periodontitis a risk factor for gastric infection? Can J Gastroenterol.;23(3):177–9.
Yilmaz O Şen N, Küpelioğlu A, Şimşek I. (2006) Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients. World J Gastroenterol September 7 Volume 12 Number 33. 33): 5375-5378
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Zhang L, Chen X, Ren B, Zhou X, Cheng L. (2022). Helicobacter pylori in the Oral Cavity: Current Evidence and Potential Survival Strategies. Int. J. Mol. Sci. 23, 1364
Sayed Abdul N, Alkhelaiwi A, Alenazi A, Alrashidi R, Salma R. (2023) The Association of Helicobacter pylori in the Oral Cavity With Dental Caries in Patients With and Without Gastric Infection: A Systematic Review. Cureus 15(5)
Jّrn A. AasAas, Ann L. Griffen, Sara R. Dardis, Alice M. Lee, Ingar Olsen Eugene J. Leys, Bruce J.(2008) Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults) JOURNAL OF CLINICAL MICROBIOLOGY,Vol. 46 no.4, p. 1407–1417
Salih A, Karakus C, Yazici D, Ulupinar Z, Akbas F, Yucel F, Akcael E, Akcan Y (2020) Development of an in-house capture ELISA: An attempt to detect CagA antigen in sera of Helicobacter pylori infected patients. Journal of Immunological Methods Volume 488.