Cytotoxic and Morphological Analysis of Azurin and Paclitaxel-Induced Apoptosis against Breast Cancer Cell line
DOI:
https://doi.org/10.51699/ijhsms.v2i8.2376Keywords:
Azurin, Paclitaxel, MCF-7 cancer cell line, Pseudomonas aeruginosaAbstract
Azurin is a cupredoxin produced by bacteria that is mostly utilized in electron transport processes. The anticancer properties of the Azurin and its potential use in cancer treatments have increased interest recently. In other side Paclitaxel is a chemotherapeutic agent used as therapy for several types of cancer, it can induce intrinsic apoptosis by activating caspase-3, caspase-9 and PARP.
Materials and Methods: The inhibitory dose 50 (IC50) of MCF-7 breast cancer cell line was assessed by MTT assay and then examined under inverted microscope to show the cytopathic effect against MCF-7 cell line.
Results: Azurin and Paclitaxel have cytotoxic effect on MCF-7 breast cancer cell line. In which, IC50 of the Azurin against MCF-7 cell line was 25.6 and IC50 of the Paclitaxel was 34.57. cytopathic effect under inverted microscope indicated that the Azurin was more cytopathic effect than Paclitaxel against the MCF-7 cell line.
Conclusion: Both Azurin and Paclitaxel have cytotoxic effect against breast cancer cells. But Azurin have more cytopathic effect than Paclitaxel.
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