Molecular Diagnosis of Sunn Pest (Eurygaster Integriceps)
Keywords:
Sunn pest, Eurygaster integriceps, Scutelleridae familyAbstract
Sun pests (Eurygaster integriceps) pose a significant threat to wheat and barley production, necessitating effective control strategies. Molecular tools like DNA barcoding and analysis of the cytochrome oxidase subunit 1 (COI) gene offer promising avenues for species identification. However, the specific application of these techniques for sunn pest identification remains underexplored. Addressing this gap, our research aimed to utilize DNA barcoding and COI gene analysis for accurate sunn pest species differentiation. Our results demonstrate that DNA barcoding is a suitable diagnostic approach, particularly through COI gene amplification, enabling reliable species identification compared to reference sequences from GenBank or Boldsystems. This finding not only fills a crucial knowledge void but also provides opportunities for tailored treatments targeting sunn pests, thereby enhancing crop protection measures.
References
R. C. Yandamuri, R. Gautam, C. Darkoh, V. Dareddy, M. El-Bouhssini, and B. A. Clack, "Cloning, expression, sequence analysis and homology modeling of the prolyl endoprotease from Eurygaster integriceps Puton," Insects, vol. 5, pp. 762-782, 2014.
G. Ferri, M. Alù, B. Corradini, M. Licata, and G. Beduschi, "Species identification through DNA 'barcodes'," Genetic Testing and Molecular Biomarkers, vol. 13, pp. 421-426, 2009.
Z. Abdul-Razak and H. F. Alrubeai, "Efficiency of pest Eurygaster Testudinaria (Geoffroy) egg parasitoids in Najaf Government," Egyptian Journal of Biological Pest Control, vol. 21, no. 2, pp. 361-368, 2011.
B. L. Parker, M. Amir-Maafi, M. Skinner, J. S. Kim, and M. El Bouhssini, "Distribution of sunn pest, Eurygaster integriceps Puton, in overwintering sites," Journal of Asia-Pacific Entomology, vol. 14, no. 1, pp. 83-88, 2011.
L. Gianessi, "Insecticide use in wheat is key for food security in the Near East and West Asia," International Pesticide Benefits Case Study, no. 81, 2013.
H. Kutuk, R. Canhilal, M. Islamoglu, A. D. Kanat, and M. El-Bouhssini, "Predicting the number of nymphal instars plus new-generation adults of the Sunn Pest from overwintered adult densities and parasitism rates," Journal of Pest Science, vol. 83, pp. 21-25, 2010.
S. Iranipour, Z. N. Bonab, and J. P. Michaud, "Thermal requirements of Trissolcus grandis (Hymenoptera: Scelionidae), an egg parasitoid of sunn pest," European Journal of Entomology, vol. 107, no. 1, pp. 47, 2010.
M. Islamoglu and S. Tarla, "Some biological properties of Trissolcus semistriatus Nees (Hymenoptera: Scelionidae) adults stored at different temperatures. 2013.
L. M. Shblawy and R. S. AL-Jorany, "Sunn pest species and its hibernation sites in Diyala governorate, Iraq," Journal of Agricultural Science, vol. 49, no. 6, pp. 1118-1122, 2018.
S. A. A. Fathi, N. Bakhshizadeh, and J. P. Michaud, "Spatial and temporal correspondence of Sunn Pest oviposition and egg parasitism by Trissolcus spp. in Iran," J. Appl. Entomol., vol. 135, pp. 666-672, 2010.
M. Mohammadpour, M. Ziaaddini, M. A. Jalali, H. Hashemirad, and A. Mohammadi-Khoramabadi, "Egg parasitoids of the pistachio green stink bug, Brachynema germari (Hemiptera: Pentatomidae) in Kerman province, Iran," Zoology and Ecology, vol. 26, no. 1, pp. 28-34, 2016.
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