Study of Cellulase Activity of Termites on Cellulose Degradation and their Inactivation with Sodium Fluoride
DOI:
https://doi.org/10.51699/ijbea.v2i12.3178Keywords:
Termite, cellulase, cellulose, endo-α-1,4-glucanases, cellobiohydrolases, β-glucosidasesAbstract
Currently, very urgent problems are the determination of the distribution of the termite population, as well as physiological and biochemical processes associated with digestive secretions and the activity of symbiont enzymes, the identification of new types of microorganisms-termite parasites, the improvement of control methods based on the creation of poisonous baits based on pathogens-fungi and microorganisms.Since termites have a specialized cellulose digestion system. Various cellulases are involved in the degradation of cellulose in termites and their symbionts. An important area of research is the study of cellulase activity of termites. The purpose of the research is to study the cellulatic enzyme activity of termites. At the same time, the activity of enzymes from different parts of the body of termites was studied. The obtained data show that the obtained suspensions from different places of the termite body after filtration hydrolyze cellulose in the form of filter paper more than suspensions without filtration, this also proves that having different substances in the substrate negatively affects the biodegradation of cellulose samples (in the form of filter paper).
References
Назаров К.К., Рахимов М.М. Изучение целлюлазной активности термитов по деградации целлюлозы // Universum: химия и биология: электроный научный журнал 2021.11(89).29-32 URL:https://7universum.com/ru/nature/archive/item/12389
Correlation of cellulase gene expression and cellulolytic activity throughout the gut of the termite Reticulitermes flavipes. Nakashima et al. 2002; Tokuda et al. 2007; Zhou et al. 2007
Watanabe H. & G. Tokuda 2010. Cellulolytic systems in insects. Annual review of entomology. 55: 609-632.
Willis J.D., C. Oppert, J.L. Jurat-Fuentes 2010. Methods for discovery and characterization of cellulolytic enzymes from insects. Insect Science 17(3): 184-198.
Tokuda G., N. Lo, H. Watanabe 2005. Marked variations in patterns of cellulase activity against crystalline- vs. carboxymethyl-cellulose in the digestive systems of diverse, woodfeeding termites. Physiological Entomology 30(4): 372-380.
Tokuda G., Lo N., Watanabe H., Arakawa G., Matsumoto T., Noda H. 2004. Major alteration of the expression site of endogenous cellulases in members of an apical termite lineage. Molecular Ecology 13(10): 3219-3228.
Nelson M.I., Kelsey R.G., Shafizaden F. Anhancement enzymatic hydroly-ses by Simultaneus attrition of cellulosed Substrates. // Bio-technol and Bioeng, 1982, vol 24,p 293-294.
Lo N., G. Tokuda, H. Watanabe 2011. Evolution and function of endogenous termite cellulases. In: D.E. Bignell et al. (eds.), Biology of Termites: a modern synthesis, pp.51- 67. Springer Science+Business Media, London New York.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
