Effect of Silymarin on the Levels of Thyroid Hormones in Rats Induced Hypothyroidism by Propylthiouracil (PTU)


  • Hawraa Aqeel Abdul Munem Department of Biology, College of Science, Al-Muthanna University, Samawah, Iraq


PTU, Silymarin, T3, T4, TSH, SOD, GSH


The purpose of this research was to identify any potential protective benefits of silymarin against the thyroid-damaging effects of PTU in male rats, with a focus on antioxidants and thyroid hormones. The following is the breakdown of the six groups comprised of four male rats with an average weight of 190 ± 10 grammes and an age of 90 days: C: For 30 days, 10 male rats in the control group were given distilled water orally. Phase 1: Ten male rats were given PTU (10 mg/kg b.w.) for 30 days. Subject 2: Ten male rats were given silymarin orally at a dosage of 200 mg/kg body weight for a duration of 30 days. T3: 10 male rats were given PTU (10 mg/kg b.w.) and Silymarin (200 mg/kg b.w.) for 30 days. Rats given PTU or silymarin had significantly different thyroid hormone levels, according to the present study. These results show that silymarin may have the ability to regulate thyroid hormone levels in hypothyroidism caused by PTU and imply that there is a complicated interaction between the three factors. To further understand how silymarin works and how long it takes to see results in treating thyroid diseases, more studies are required. Research also shows that silymarin may help with PTU-induced hypothyroidism by increasing GSH levels, decreasing lipid peroxidation, and improving antioxidant enzyme activity. One possible way to combat the oxidative stress that hypothyroidism causes is to take silymarin supplements. To further understand how silymarin works and how long it takes to see results in treating thyroid diseases, more studies are required.


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L. F. L. Rizzo, D. L. Mana, and H. A. Serra, “Drug-induced hypothyroidism,” Medicina (Buenos Aires), 2017, [Online]. Available: http://www.scielo.org.ar/scielo.php?pid=S0025-76802017000500008&script=sci_arttext

P. N. Taylor, D. Albrecht, A. Scholz, and ..., “Global epidemiology of hyperthyroidism and hypothyroidism,” Nature Reviews …, 2018, [Online]. Available: https://www.nature.com/articles/nrendo.2018.18

V. Křen and D. Walterová, “Silybin and silymarin-new effects and applications,” Biomed papers, 2005, [Online]. Available: https://biomed.papers.upol.cz/pdfs/bio/2005/01/02.pdf

C. A. Burgess, Silybum marianum (milk thistle). sid.ir, 2003. [Online]. Available: https://www.sid.ir/paper/532916/en

W. Borymska, M. Zych, S. Dudek, and I. Kaczmarczyk-Sedlak, “Silymarin from milk thistle fruits counteracts selected pathological changes in the lenses of type 1 diabetic rats,” Nutrients, 2022, [Online]. Available: https://www.mdpi.com/2072-6643/14/7/1450

S. Meng, “Silymarin ameliorates diabetic cardiomyopathy via inhibiting TGF-β1/Smad signaling,” Cell Biol Int, vol. 43, no. 1, pp. 65–72, 2019, doi: 10.1002/cbin.11079.

M. López-Torres, M. Romero, and G. Barja, “Effect of thyroid hormones on mitochondrial oxygen free radical production and DNA oxidative damage in the rat heart,” Mol Cell Endocrinol, 2000, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0303720700003026

E. Pucci, L. Chiovato, and A. Pinchera, “Thyroid and lipid metabolism,” Int J Obes, 2000, [Online]. Available: https://www.nature.com/articles/0801292

D. J. Selvage and C. A. Johnston, “Interaction between norepinephrine, oxytocin, and nitric oxide in the stimulation of gonadotropin‐releasing hormone release from proestrous rat basal hypothalamus …,” J Neuroendocrinol, 2004, doi: 10.1111/j.1365-2826.2004.01235.x.

J. Całka, “The role of nitric oxide in the hypothalamic control of LHRH and oxytocin release, sexual behavior and aging of the LHRH and oxytocin neurons.,” Folia Histochem Cytobiol, 2006, [Online]. Available: https://journals.viamedica.pl/folia_histochemica_cytobiologica/article/view/4581

M. Osuchowski, V. Johnson, Q. He, and ..., “Alterations in regional brain neurotransmitters by silymarin, a natural antioxidant flavonoid mixture, in BALB/c mice,” Pharmaceutical …, 2004, doi: 10.1080/13880200490519712.

H. G. Oufi, N. N. Al-Shawi, and S. A. R. Hussain, “What are the effects of silibinin on testicular tissue of mice?,” Journal of Applied …, 2012, [Online]. Available: http://japsonline.com/abstract.php?article_id=688

A. Ceriello and R. Testa, “Antioxidant anti-inflammatory treatment in type 2 diabetes,” Diabetes Care, 2009, [Online]. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811469/

X. Wu, G. R. Beecher, J. M. Holden, D. B. Haytowitz, and ..., “Hydrophilic Antioxidant Capacities of Common Foods in the United States., 2004, 52,” DOI: https://doi. org/10.1021 ….

R. Shukla, S. Gupta, J. K. Gambhir, K. M. Prabhu, and ..., “Antioxidant effect of aqueous extract of the bark of Ficus bengalensis in hypercholesterolaemic rabbits,” Journal of …, 2004, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0378874104000613

H. Wiseman, “Dietary influences on membrane function: importance in protection against oxidative damage and disease,” J Nutr Biochem, 1996, [Online]. Available: https://www.sciencedirect.com/science/article/pii/0955286395001522

A. Schuh, “Presentation of Graves’ orbitopathy within European Group On Graves’ Orbitopathy (EUGOGO) centres from 2012 to 2019 (PREGO III),” British Journal of Ophthalmology, vol. 108, no. 2, pp. 294–300, 2023, doi: 10.1136/bjo-2022-322442.

Y. Horie, “Adverse effects of thyroid-hormone-disrupting chemicals 6-propyl-2-thiouracil and tetrabromobisphenol A on Japanese medaka (Oryzias latipes),” Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 263, 2023, doi: 10.1016/j.cbpc.2022.109502.

I. Rani, “An Appraisal on Synthetic and Medicinal Aspects of Fused Pyrimidines as Anti Neoplastic Agents,” Anticancer Agents Med Chem, vol. 23, no. 5, pp. 525–561, 2023, doi: 10.2174/1871520622666220701113204.

P. Pannetier, “Reversibility of Thyroid Hormone System–Disrupting Effects on Eye and Thyroid Follicle Development in Zebrafish (Danio rerio) Embryos,” Environ Toxicol Chem, vol. 42, no. 6, pp. 1276–1292, 2023, doi: 10.1002/etc.5608.

S. Alam, “Antidiabetic Phytochemicals From Medicinal Plants: Prospective Candidates for New Drug Discovery and Development,” Front Endocrinol (Lausanne), vol. 13, 2022, doi: 10.3389/fendo.2022.800714.

B. Akbari, “The role of plant-derived natural antioxidants in reduction of oxidative stress,” BioFactors, vol. 48, no. 3, pp. 611–633, 2022, doi: 10.1002/biof.1831.

C. H. Wong, “Effect of Inactivated and mRNA COVID-19 Vaccination on Thyroid Function among Patients Treated for Hyperthyroidism,” Journal of Clinical Endocrinology and Metabolism, vol. 108, no. 5, 2023, doi: 10.1210/clinem/dgac684.

S. Y. Lee, “Comparison of Propylthiouracil vs Methimazole for Thyroid Storm in Critically Ill Patients,” JAMA Netw Open, vol. 6, no. 4, 2023, doi: 10.1001/jamanetworkopen.2023.8655.

B. M. Sahoo, “Microwave Induced Green Synthesis: Sustainable Technology for Efficient Development of Bioactive Pyrimidine Scaffolds,” Curr Med Chem, vol. 30, no. 9, pp. 1029–1059, 2023, doi: 10.2174/0929867329666220622150013.

N. A. N. Hanafy, “Silymarin/curcumin loaded albumin nanoparticles coated by chitosan as muco-inhalable delivery system observing anti-inflammatory and anti COVID-19 characterizations in oleic acid triggered lung injury and in vitro COVID-19 experiment,” Int J Biol Macromol, vol. 198, pp. 101–110, 2022, doi: 10.1016/j.ijbiomac.2021.12.073.

K. U. Khan, “Overview of nanoparticulate strategies for solubility enhancement of poorly soluble drugs,” Life Sci, vol. 291, 2022, doi: 10.1016/j.lfs.2022.120301.

A. Chauhan, “Phytochemicals targeting NF-κB signaling: Potential anti-cancer interventions,” J Pharm Anal, vol. 12, no. 3, pp. 394–405, 2022, doi: 10.1016/j.jpha.2021.07.002.

S. Fakhri, “Modulation of dysregulated cancer metabolism by plant secondary metabolites: A mechanistic review,” Semin Cancer Biol, vol. 80, pp. 276–305, 2022, doi: 10.1016/j.semcancer.2020.02.007.

J. W. Jang, “KASL clinical practice guidelines for management of chronic hepatitis B,” Clin Mol Hepatol, vol. 28, no. 2, pp. 276–331, 2022, doi: 10.3350/cmh.2022.0084.




How to Cite

Munem, H. A. A. . (2024). Effect of Silymarin on the Levels of Thyroid Hormones in Rats Induced Hypothyroidism by Propylthiouracil (PTU). INTERNATIONAL JOURNAL OF HEALTH SYSTEMS AND MEDICAL SCIENCES, 3(2), 1–7. Retrieved from https://inter-publishing.com/index.php/IJHSMS/article/view/3473