The Philadelphia Chromosome Detection in Chronic Myeloid Leukemia in Karbala City
Keywords:Chronic myeloid leukemia, BCR-ABL, Philadelphia chromosome
Background: The mutual transposition t (9;22) (q34; q11) results in the Philadelphia chromosome (Ph), which is an ideal marker of chronic myeloid leukemia (CML). The proto-oncogene tyrosine-protein kinase (BCR-ABL1) oncogenic breakpoint cluster region-protein with enhanced tyrosine kinase action is encoded by this fusion gene. The kinase movement is measurable for cell proliferation, diversity inhibition, and cell death confrontation. Styles of representation in isolate BCR-ABL1 transcripts change when CML progresses transitioning from a chronic to an expedited phase and ultimately to the blast phase. Every BCR-ABL1 transcription is concomittant with a specific leukemia phenotype that expects treatment clinical outcome and reaction prognosis. The Ph is seen in acute lymphoblastic leukemia, acute myeloid leukemia, and motley-type acute leukemia, in addition to CML.
Aim of the study: to give a view of the clinical diagnostic importance of Philadelphia chromosome CML and role of Philadelphia in comparison to morphology.
Methods: A total of 180 patients had developed leukocytosis with severe Lf-shift by blood film. Data for patients, as age and sex, were collected. Blood samples, including CBC with total WBC, and differential counts, were performed using 5-parts differential URIT autoanalyzer hematology.
Results: A total of 180 patients with leukocytosis, 150 of them had Philadelphia chromosome-positive with a mean age of 52.86 ± 13.9 (range: 29-80) years were collected. Sixty (50%) cases were male and sixty % were female and 30 cases of them were Philadelphia negative with a mean age of 56.1 ± 14.8 years, 17 cases were male and 13 cases were female. There was an important difference between the two groups. In this study, the mean ±SD for Philadelphia positive patients was found to be a very highly significant difference when compared with Philadelphia negative group, at the level of significance (<0.001) by student T-Test.
Conclusion: The research of myeloproliferative disorders has many goals: diagnostic, therapeutic, predictive and follow-up, all of which contribute to better outpatient therapy.
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