Isolation and Molecular Detection of Some Anaerobic Bacteria From Patients With Periodontitis
DOI:
https://doi.org/10.51699/ijhsms.v2i8.2432Keywords:
Anaerobic, Periodontitis, bacteria, MolecularAbstract
This study aimed conducted for isolation and Molecular detection of some anaerobic bacteria from patients with Periodontitis. Transport and collection: 78 Patients with periodontitis between the ages of 16 and 69 from Dental Clinics in the city of Baghdad were recruited between 2021 and 2022 for this study. After placing a paper point (size 30-45 mm.) inside a pocket with a depth of 3 mm. and leaving it there for 60 seconds, we were able to obtain an adequate sample of GCF for analysis. Next, Thioglycollate broth was added to the paper point. Within 2 hours of collection, all samples were sent to the bacteriology lab where they were cultured at 37 °C for 24-48 hours. Genomic DNA micro Kit was used to isolate the bacterial genome. Amplification using global bacterial 16S rDNA primers. From a total of 78 samples, 34 unique anaerobic bacteria were isolated. Bacteriological techniques were used to identify the five species of bacteria (representing four genera) that were isolated. The Gramme stain test was used for initial confirmation of the microbiological isolates found in both diabetes and non-diabetic individuals, and the findings showed that 24 of the bacterial isolates were Gramme positive cocci, 5 were Gramme positive anaerobic bacilli, and 3 were Gramme negative anaerobic Cocci. The results displays the gender breakdown of our patient population, which breaks down to 15 male (42.9% of the total) and 25 females (58.1% of the total), with no statistically significant differences (P > 0.05) between the sexes. Streptococcus salivasrius was found to be the most resistant species in this study, accounting for 18 (52.9%) of the isolates, followed by Lactobacillus salivarius with 7 (20.6%) isolates, Streptococcus mutans with 5 (14.7%), and Veillonella Spp. with 2 (5.5%). However, only 2.5% of diabetes individuals have the bacterium species Enterococcus faecalis. All these bacteria were confirmed by using PCR.
In conclusion, the present investigation found that Streptococcus salivarius was the most resistant in all individuals, followed by Lactobacillus salivarius.
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