Estimation level of blood clotting factors (F7, F8,F9) and Interleukin (IL1b,IL-37) in patient with Hemophilia through gene Micro RNA145
Keywords:
Clotting Factor, Hemophilia, IL1b, IL-37, Mirna145Abstract
This study was conducted with the aim of knowing the correlation between blood clotting factors (F9, F8, F7), immune factors, interleukins (IL1b, IL-37) and the gene (Micro RNA145) in the blood of patients with hemophilia. Samples were taken from several hospitals in Iraq (Ramadi Hospital For women and children in Anbar Governorate, Tikrit General Hospital in Salah al-Din Governorate, Child Protection Hospital in Baghdad, Baghdad Teaching Hospital laboratories), where the study included 65 samples from patients with hereditary hemorrhage of various types (HA, HB, HC, and W.V.P). The study measured the levels of clotting factors (F9, F8, F7) using the ELISA technique, and investigated the gene expression levels of Micro RNA145 using qRT-PCR technology by converting it to cDNA. The results of the current study showed that the seventh clotting factor, F7, has a significant decrease in its percentage in patients compared to its percentage in healthy people, while the eighth blood clotting factor, F8, has no significant difference. As for the ninth blood clotting factor, F9, a significant decrease in the factor values was found. In infected patients compared to healthy people, the current study also showed that there was a significant decrease in the level of values of interleukin IL-37 in patients compared to the blood of healthy people. As for interleukin IL1b, the results of the current study showed that there was a significant increase in the values of the factor in patients with hemophilia compared to healthy people. As for the results of the genetic analysis, it was not There is a significant difference between the studied gene and the factors (8,9). The study found connections between variables and the expression of the MIR145 gene. Factor 7 was found to be positively correlated with Factors 8 and 9, suggesting potential shared biological influences. Elevated IL 37 levels coincided with increases in Factors 7 and 9, while IL 1b levels were linked to Factors 7 and 8. The links between MIR145 gene expression and Factors 7 and 9 were weaker, suggesting an association between MIR145 expression and these factors. No significant correlation was found between IL 37 and MIR145 gene expression.
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