Effect of Adding Different Levels of Moringa oleifera Leaves Extract to Drinking Water on Some Physiological traits of Broiler (Ross 308)


  • Batoul Abed_alnaby Shaker Alfurat Al-Awsat Technical University, Kufa
  • Nihad Abdul-Lateef Ali AL-Qasim Green University2, Babylon, Iraq


Nano-Alcoholic Extract Of Moringa Oleifera Leaves, Physiological Characteristics, Broiler Chickens


The Department of Animal Production at the College of Agriculture / Al-Qasim Green University conducted this experiment in the poultry farm to examine the effects on a few physiological traits of broiler chickens of adding varying levels of Moringa oleifera leaf nano-alcoholic extract (MOLE) to drinking water. 180 unsexed one-day-old Ross broiler chicks were utilized. Four groups  were randomly assigned to them, 45 birds were used for each group, and each group was repeated three times (15 birds each replicate). The following were the experimental groups : the initial group (control), which involved not putting (MOLE) in the drinking water. The second group involved adding 10 milliliters (ml) of 0.02% level (MOLE) per liter of drinking water. The third group was the addition of a 10 ml/liter dose of a 0.04% level of (MOLE). The fourth group involved adding a 10 ml/liter dose of a 0.06% level of (MOLE). The following characteristics were examined in the experiment: hematological characteristics, including the number of red and white blood cells, packed cell volume, and hemoglobin level; biochemical characteristics, including all of the levels of glucose, cholesterol, triglycerides, globin, albumin, and total protein; and immunological characteristics, the delayed hypersensitivity test in wattles, relative weight, and evidence of Fabricia's gland. The findings show that, when compared to the other research groups , the addition of (MOLE) at a level of 0.06%/liter of drinking water significantly improved all evaluated attributes (P≤0.05).


Al-Dafai, Hossam Abdel-Wahab. 2000. Use of double and single inoculation of Camboro and Newcastle vaccines in broiler chickens. Master Thesis . College of Veterinary Medicine. Baghdad University.

Al-Hassani, Dhia Hassan. 2000. Chicken poultry farm. Dar Al-Kutub for Printing and Publishing. Baghdad.

AL-Murrani, W.K., H. Hamed,Z. G. Abdul-Gani and A.H. Omran. 1995. Someaspects of genetic ressistance to S.typhimurium in native Iraqi and white leghorn chickens. Dirasat . 22(2) .Jordon.

Aqeel. Y. A. ; Nihad A. Ali ; Rabia J. Abbas ; Ali M. Alkassar and Yasser J. Jameel.2018. Effect of Dietary Supplementation with Differing Levels of Moringa oleifera Leaf Meal on the Productivity and Carcass Characteristics of Broiler Chickens.Int.J.poult.Sci.,17: 536- 542 .

Archer, R.K., 1965. Hematological Technique for use on animals. Black Well Scientific Publications, Oxford.

Bukar, A; A. Uba and Oyeyi, T. I. 2010. Antimicrobial profile of Moringa oleifera Lam. extracts against Mal. J. Microbiol., 8(2): 59-67.

Burton, R.R. and C.W. Guion.1968.The Differential leukocyte blood count. Its precision and individuality in the chicken. Poultry Sci. 47: 1945-1949.

Donkor A M, Kwame Glover R L, Addae Daniel and Kubi K A. 2013. Estimating the nutritional value of the leaves of Moringa oleifera on poultry. Food and Nutrition Sciences 4: 1077– 83.

Doughari, J.H.; M.S. Pukuma and De. N. 2007. Antibacterial effects of Balanites aegyptiaca L. Drel. and Moringa oleifera Lam. on Salmonella typhi. African. J. Biotechnol., 6(19): 2212-2215.

Duncan , D. B. 1955 . Multiple ranges test and Multiple F – test . Biometrics . 11: 1 – 42.

Feynman , R. 1959 . Plenty of room at the bottom . American Physical Society at Caltech food: a Nano forum Report. Nano Forum .org

Kuhn, E.R., L.R. Berghman, L. Moons, F. Vandeande ,E. Decyper and V.M. Darres.1993. Hypothalamic and peripheral control of thyroid function during the life cycle of The chicken In: Avian Endocrinology. Edt. Sharp, P. J. Journal of Endocrinology Ltd., Bristol.

LEAVES TO DRINKING WATER ON THE PRODUCTIVE PERFORMANCE OF LAYING HENS. MINAR International Journal of Applied Sciences and Technology.4(2):25-40 .

Lucio, B., and S.B. Hitchner .1979. Response of susceptible versus immune chickens to infections bursal disease virus Vaccine. Avian Dis. 23:1037-1049.

Maxwell MH., GW. Robertson and CC. McCorquodale 1992. Whole blood and plasma viscosity values in normal and ascitic broiler chickens. British Poultry Science. Volume 33, Issue 4, Pages 871-877 .

Monika BT, RYK Roman, S Zymeczko and AP Iotrowski 2012. The Level of Major Proteins and Minerals in the Blood Serum of Chickens Fed Diets with Pure Cellulose. Institute of Systematics and Evolution of Animals. vol. 60 , No 1-2.

Murro J K, Muhikambele V R M and Sarwatt S V. 2003. Moringa oleifera leaf meal can replace cottonseed cake in the concentrate mix fed with Rhodes grass (Chloris gayana) hay for growing sheep. Livestock Research for Rural Development.Volume 15, Article 84.

Najafi P, Torki M. 2010. Performance, blood metabolites and immunocompetaence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances. 9:1164-1168.

National Research Council (NRC) . 1994. Nutrient requirement of poultry then. National Academy press. Washington. D. C. USA .

Natt MP and CA. Herrick 1952. A New Blood Diluent for Counting the Erythrocytes and Leucocytes of the Chicken Poultry Science, Volume 31, Issue 4, 1 Pages 735–738.

Nazmy, S., Hassan, B., Nihad, A. A., Abeer, E., Abd Elhamid, E and Farid, M. 2016. Biochemical Studies on Moringa Oleifera Leaves Extract. Journal of Biology, Agriculture and Healthcare. Vol.6, No.16 .

Nbras Kadhim Abbas and Nihad Abdul-Lateef Ali .2019. Effect of adding different levels of nano-boron particles to the diet in some of the productive traits for the broiler chicken (Ross 308). Plant Archives Vol. 19 No. (2) : 3446-3452

Nihad A. A. Alnidawi ; Hanaa, F. M. Ali ; Sherein, S. Abdelgayed ; Fatma, A. Ahmed and M. Farid.2016. Moringa Oleifera Leaves in Broiler Diets: Effect on Chicken Performance and Health. Food Science and Quality Management.,58:40-48

Nihad A. Ali ; Fadhil R. A. ; Imad A. and Ammar S. AL- Nassry.2019. Effect of adding white tea powder (camellia sinensis)to the ration in the qualitative traits of Japanese quail eggs (Coturnix coturnix japonica) . Bioscience Research, 16(1) : 459-464.

Nihad A. Ali ; Galib A.AL-Kaissy; Mamdooh A. M. AL- Nasrawi.2016. Effect of Addition Different Levels of Parsley Leaves Powder (petroselinum sativum) to the Ration on Some Blood Serum Biochemical Traits of Broiler Ross 308. Journal of Natural Sciences Research. Vol.6, (20) : 18-21 .


Olugbemi TS, Mutayoba SK, Lekule FP.2010. Evaluation of Moringa oleifera leaf meal inclusion in cassava chip based diets fed to laying birds. Livestock Research for Rural Development. 22(6).

Oraibi , Dakhil Hasan and Ali, Nihad Abdul –Lateef .2021. Effect of Adding Alcoholic and Nano Alcoholic Extract Of Moringa Oleifera Leaves to Drinking Water on the Biochemical Blood Traits for Laying Hens Lohmann Brown. Indian Journal of Forensic Medicine & Toxicology, July-September , Vol. 15, No. 3 .

SAS, 2012. SAS/ STAT Users Guide for Personal Computers Release 9.1 SAS . Institute Inc. Cary and N.C USA .

Troncarelli, M. Z. ; Brandão, H. M., Gern, J. C., Guimarães, A. S., and Langoni, H. 2013 . Nanotechnology and antimicrobials in veterinary medicine.Badajoz, Spain:FORMATEX University of Nebraska. Linolin, Lincd, NE with acute myocardial infraction". National J. of chemistry. ; (4): 625-637.

Varley, H., A. H. Gowenlock and M. Bell 1980. Practical clinical Biochemistry. 5th ed. William Heinemann Medical Books LTD. ,London.

Voller, A., D.E.Bidwell and A.Bartlett .1977. The Enzyme Linked Immunosorbent Assay (ELISA). pp. 24-26. Flow-line Publications, Guernsey.

Wooton IDP 1964. Micro-analysis in Medical Biochemistry. Fourth edition. pp.114-118. Churchill: London.

Youssef A. A., A. A. Bakhashwain and N.K. Bertu. 2017. Thyme oil (Thyme vulgaris L.) as a natural growth promoter for broiler chickens reared under hot climate. Italian Journal of Animal Science. Volume 16, Pages 275-282.




How to Cite

Shaker, B. A., & Ali, N. A.-L. (2024). Effect of Adding Different Levels of Moringa oleifera Leaves Extract to Drinking Water on Some Physiological traits of Broiler (Ross 308). International Journal of Biological Engineering and Agriculture, 3(3), 247–254. Retrieved from https://inter-publishing.com/index.php/IJBEA/article/view/3568