Detection of The Effect of Deltamethrin On Gene of Cucumber Plants Using RAPD-PCR
DOI:
https://doi.org/10.51699/ijbea.v2i9.2480Keywords:
detection, deltamethrin, gene, plantsAbstract
The current study was conducted to find out the effect of some insecticides on the genetic characteristics of the plant that parasitized it, where the study included the cultivation of cucumber plant on previously prepared land in the form of repeaters, and the number of repeaters was 10 times, and in each repeater, ten seedlings of the plant were placed, then each repeater was covered with tulle cloth Transparent through which air and water pass only. The plant was treated with a chemical pesticide (Deltathor) from the American company Ensysetx and with the doses on the cover of the box, with three treatments between each other treatment for 20 days. Samples were taken from the plant after each treatment, DNA was extracted from it, the genetic changes were known and compared with the control sample. Where RAPD-PCR and Sequence technology were used to identify these genetic changes and mutations.
The results showed that the primers of the deltathor pesticide at three concentrations used on the cucumber plant were different bands, as they showed different patterns of bands, as if the total number of bands recognized by the primers on the sample gene was 258 bands, and the total distinct bands resulting from the primers of this pesticide were 157 bands, as if 86 of them were bands. unique and 71 absent bands, and the primer 6 P and p4 were characterized by the highest number of bands produced, which amounted to 66 bands, while the two initiators P5 produced the lowest number of bands, which amounted to 16 bands
Where the results of the primers showed a clear discrepancy, as it ranged between 200-1700 bp, as if the lowest molecular size was 200 bp in the primer 2P and p4, and the highest molecular size was in each of the two primers P3, 6p, as the molecular size reached 1700 bp for each of them. The samples treated with the pesticide were 100% characterized by the highest percentage. There were 58 distinct packages, followed by samples treated with pesticide 50%, which produced 53 packages, followed by samples treated with pesticide 25%, which produced 46 distinct packages.
The results of Sequence technology showed exactly the same results as the results of RAPD-PCR. Where it indicated in the genome of the cucumber plant, it indicated that the effect of deltathor is a strong pesticide.
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