Response of Various Lettuce Varieties (Lactuta Sativa L.) to Fe Micro Fertilizer in Hydroponic Cultivation Systems

Lettuce is a type of vegetable plant whose leaves are consumed. The prospect of market uptake of lettuce commodities will continue to increase in line with the increase in population. Micronutrients in plants play a very important role in plant production and productivity. Among the micronutrients found in plants is the nutrient element Iron (Fe). Iron (Fe) is a cofactor of around 140 enzymes which function to analyze biochemical reactions and is a very important element for plant growth. This research aims to design a technology package for cultivating lettuce plants through Integrated Plant Management (PTT). This research used a split plot design based on a Completely Randomized Design (CRD) with three replications consisting of two treatment factors. Namely the first factor as the main plot, namely: providing the type of nutrition (N) and the second factor as the sub-plot, namely: variety. The response of various lettuce varieties to various Mikto Fe fertilizer applications had a significant effect on plant height, leaf width, leaf length, number of leaves and root length. Red and Romaine lettuce varieties are lettuce varieties that respond positively to Micro Fe fertilizer treatment. The provision of various Fe micronutrients to various lettuce varieties had a significant effect on all observed parameters. EDDHA Single Fe micronutrient is the best Fe micronutrient. The interaction between lettuce varieties and Fe micronutrients did not provide much interaction with the observed parameters, only the root length parameter showed a real influence. The interaction of Romaine lettuce varieties with EDDHA Single Fe microorganisms tends to provide the best results


INTRODUCTION
Lettuce is a type of vegetable plant whose leaves are consumed.The prospect of market uptake of lettuce commodities will continue to increase in line with the increase in population, increase in public education, increase in income and welfare of the community, and increase in people's preferences for lettuce (Samadi, 2014).Lettuce is a vegetable that belongs to the Asteraceae family and has high economic value.Lettuce contains the minerals iodine, phosphorus, iron, copper, cobalt, zinc, calcium, manganese and potassium so it is efficacious in maintaining body balance (Aini et al., 2010).Lettuce has a crunchy texture and a variety of leaf colors.Every 100 grams of wet lettuce contains 1.2 g protein, 0.2 g fat, 22.0 mg Ca, 22.0 mg Fe, 162 mg Fe, 0.04 mg vitamin A, 8.0 mg vitamin C (Wardhana, et al., 2017).
Micronutrients in plants play a very important role in plant production and productivity.Among the micronutrients found in plants is the nutrient element Iron (Fe).Iron (Fe) is a co-factor of around 140 enzymes which function to analyze biochemical reactions (Brittenham, 1994) and is a very important element for plant growth.Iron deficiency causes young leaves to turn yellow and photosynthetic activity is reduced significantly and as a result of iron (Fe) deficiency biomass is reduced (Briat, et al, 2007 andHochmuth, 2015).Iron plays many important roles in plant growth and development, including chlorophyll synthesis, thylakoid synthesis, chloroplast development, contributing to RNA synthesis and improving photosystem performance (Miller, et al., 1995 andSheykhbaglou, et al., 2010).The element iron (Fe) is abundant, because it is inaccessible, causing plants to lack Fe.Most iron compounds are insoluble compounds, and only a small amount of iron is soluble (Fe(OH)2, Fe(OH)+2, Fe+3, Fe+2).
The amount of this soluble form of iron depends on pH so that it is maximum at acidic pH and minimum in the pH range between 6.5 and 7.5 (Kangueehi, 2008).Abbas et al. (2009) applied 0, 4, 8, 12 and 12 kg ha-1 in the form of iron sulfate to the soil and showed that iron fertilization increased the Fe and protein content of wheat grains.With the application of 150 g ha-1 iron in the form of Fe2O3, Habib (2009) reported that the iron and protein content of wheat grain was increased.Zeidan et al. (2010) applied foliar Fe fertilizer (1.0%FeSO4) and reported that Fe application increased the protein and Fe content of wheat seeds.Liu, et al. (2005), reported that nano-Fe2O3 promoted the growth and photosynthesis of peanuts.Sheykhbaglou, et al, 2010 showed that the application of nano-iron oxide particles increased soybean yield.Prasad, et al., (2010) reported that nanoscale zinc oxide particles increased stem and root growth and peanut pod yield compared to ZnSO4 application.The use of various types of fertilizer that are appropriate and useful is the main way to reform and maintain soil fertility and increase crop yields (Rezaeei, et al., 2014).Metal chelate fertilizers can include many different chemicals that are usually referred to by their abbreviated names and include EDTA (ethylene-diamine-tetra-acetic acid), DTPA (diethylene-triamine-pentaacetic acid), or EDDHA (ethylene-diaminedi o-hydroxyphenylacetic). sour).All of these materials are water soluble, but it is important to choose the right one because they have different abilities to form stable metal complexes depending on the soil pH.For example, EDTA preferentially chelates calcium over iron or zinc at neutral to alkaline pH (pH > 7.0).For iron fertilization, the best chemical is EDDHA (Sequestration 138).This metal chelator is very stable and can persist for several years in the soil because it recycles many times to dissolve more iron in the soil and deliver it to the roots.The amount of trace metal fertilizer used depends on the type of fertilizer material and the severity of the deficiency (David, et al., 2001).Welch and Graham [2002] suggest that Fe deficiency in wheat grain can be overcome by breeding and selecting cultivars that can absorb more Fe from the soil and accumulate it in the grain, while Yip (1997) argues that Fe deficiency can be overcome by food fortification.However, plant breeding is time consuming and iron fertilizer applied to plants by this method can reach target plants well below the minimum effective concentration.In addition, the effectiveness of Fe 81 fertilizers (FeSO4, FeEDTA, FeDTPA, FeEDDHA, Fe-citrate) in overcoming Fe deficiency varies greatly depending on their solubility, stability, ability to penetrate through the leaf cuticle, mobility and following translocation.diffusion into the leaf tissue (Schonherr, et al., 2005 andTabrizi, et al., 2009.Iron chelates based on EDDHA are stable in the soil and prevent iron deposition for a reasonable period of time.EDDHA chelation agents store ferrous iron at high levels and prevent its deposition in soil.Thus the concentration of iron in the soil increases but this fertilizer has the problem that it is very expensive (Harsini, et al., 2014).Iron compounds can be used as leaves on leaves and as seed coatings.With the production of nano fertilizer, these nano compounds can quickly and completely absorbed by plants and corrects nutritional deficiencies and needs (Harsini, et al., 2014).The use of nano fertilizers causes increased element efficiency, reduces soil toxicity, at least achieves the negative effects caused by excessive fertilizer consumption and reduces the frequency of fertilizer application (Harsini, et al., 2014) organic and mineral materials.This fertilizer is fully compatible with the environment and agriculture and organic matter by adding to the soil to make it more organic matter (Harsini, et al., 2014).The results of comparing the effect of nano Fe chelate with Fe chelate on the growth parameters of Ocimum basilicum show that the replacement of iron fertilizer produced with nanotechnology compared to ordinary Fe fertilizer can increase the quantitative and qualitative growth of plants in appropriate concentrations or less (Zhu, et al., 2011).Regarding leaf Fe concentration, it can be seen that the effect of leaf FeSo4 on leaf Fe concentration is higher than Fe-EDTA in Strawberry cultivars (Harsini, et al., 2014).Nanotechnology can provide solutions to increase the value of agricultural products and reduce environmental problems.By using nanoparticles and nanopowders, we can produce controlled or delayed release fertilizers.Nanoparticles have high reactivity due to a more specific surface area, more density of reactive areas, or increased reactivity of these areas on the particle surface.These features make it easier to absorb fertilizers and pesticides produced at the nanoscale (Zhu, et al., 2011).Studies show that the effects of nanoparticles on plants can be beneficial (growth and development of seedlings (Zhu, et al., 2008).

Time and Place of Research
This research was conducted at the Research House located on Jalan Ngatmorejo, Mandaran Hamlet, Puger Wetan Village, Puger District, Jember Regency.Research implementation will begin from March to May 2022 with an altitude of 20 above sea level (asl).

Research Materials and Tools
The materials used in this research were curly lettuce seeds, red lettuce, romaine lettuce, EDTA single micro Fe fertilizer, EDDHA single micro Fe, compound micro Fe, rockwooll, flannel cloth, well water.The tools used in this research were netpots, plastic cups, plastic measuring cups, mini hacksaws, nitric acid, buckets, hydroponic installation circuits, pumps, TDS meters, pH meters, rulers and digital scales.The planting media in this research used rockwool, netpot, flannel cloth, and AB mix nutrients.

Experimental design
This research used a split plot design arranged based on a Completely Randomized Design (CRD) with three replications consisting of two factors.The first factor as the main plot was: provision of type of nutrition (N), consisting of 3 levels, namely: N1 = Single Fe EDDHA, N2 = Single Fe EDTA, N3 = Compound Fe and) The second factor, as a subplot, namely the type of variety (V) consists of 3 levels: V1 = Curly Lettuce, V2 = Red Lettuce, and V3 = Romaine Lettuce.The Treatment Lay Out is as follows.The experimental chart and randomization of treatments can be seen in Figure 1  The analytical method used in this research uses ANOVA analysis of variance.If the results have a real effect then continue with the Duncan test (DMRT).The data analysis method for a 2-factor completely randomized design is as follows: Yijk = µ + αi + βj + γik + (αβ)ij + €ijk Information: Yijk = Observations on the kth experimental unit that received a combination of treatment at the ith level of factor N and the jth level of factor V, µ = True average value (population average), αi = Additive effect of the th level -i of factor N, βj= Additive influence of the jth level of factor V, (αβ)ij = Additive influence of the ith level of factor N and jth level of factor V, γik = Random effect of the main plot, which arises from the ith level of factor A in the kth replication, €ijk = Random effect of the kth experimental unit that received the treatment combination ij.

Research Implementation
This research began with preparation for the installation of the research site, namely making a hydroponic rack.The hydroponic rack uses 3" PVC pipe gavalum, the distance between holes is 16.2 cm, each rack has 36 planting holes, this research uses three racks as replications (figure 1).The plant nursery begins by cutting the rockwool using a hacksaw with a small section size of 1.5 x 1.5 x 1.5 cm.Before sowing the cut rockwooll, water it until it is wet, then make a planting hole the size of a lettuce seed, then fill each hole in the rockwoll with 1 lettuce seed.Then observe the seeds every day.If there are any seeds that break/sprout, they can be moved to a place exposed to sunlight, keep the rock wool always wet and do this process until 4 lettuce leaves ready to be transplanted into the hydroponic installation that has been prepared.AB mix nutrients are in granular form consisting of stock A and stock B solutions, each weighing 125.There are 3 AB mix nutrients which have different micro Fe content, namely: single Fe EDDHA, Fe EDTA and compound micro Fe.Provide 2 bottles of 1 liter size, to make the dissolving process easier, also provide 2 buckets.then each bucket with 500 ml of raw water, put the stock A and stock B solutions into the bucket provided, stir until completely dissolved in the water.After that, put stock A and B in a 1 liter bottle and add more raw water until each becomes 1 liter.After that, enter stock A and stock B solutions, 45 ml each, into a nutrient water tank containing 15 liters.AB mix nutrition is given at the beginning of filling the nutrient water tank and if the nutrient water tank decreases by giving an AB mix concentration of 800 ppm.The observation variables observed include the following: 1. Length of leaves (cm), 2. Number of leaves (pieces).3. Leaf width (cm), 4. Plant height (cm), 5. Root length (cm), 6. Root fresh weight (g), 7. Plant fresh weight (g)

RESULTS AND DISCUSSION
Research result about Response various varieties lettuce (Lactuta sativa L.) Against fertilizer micro fe on the system cultivation in a way hydroponics using the parameters plant height, number leaves, long leaf.Heavy leaves, root length, fresh weight of roots, fresh weight of planting.As for the results analysis The variety of each observation variable is in table 1 Based on results analysis variety in on treatment nutrition (N) show different very real in all observation parameters except for the width parameter leaf aged 15 years old show significant influence, moderate influence variety (V) indicates diverse influences and influences interaction between nutrition with variety (NxV) indicates influence No different real, except for the root length parameter show real influence (Table 1)

Plant Height
Results analysis variety to increase tall plant lettuce show that treatment nutrition very significantly different across ages plant 15 and 45 HST.Whereas interaction between nutrition and varieties different No real on all age plants (Table 1).3. On age 15 hst varieties lettuce romaine (V3) produce average plant highest namely 7.65 cm followed by varieties lettuce red (V2) and varieties Lettuce curly (V1).At the age of 30 HST varieties romaine lettuce (V3) yields an average crop highest namely 16.19 cm followed varieties Lettuce red (V2) And varieties Lettuce curly (V1), whereas on age 45 hst varieties Romaine lettuce (V3) yields an average crop highest ie 28.61 cm followed varieties Lettuce red (V2) and varieties Lettuce curly (V1) (Table 3).Matter This show that varieties Romaine lettuce (V3) is more responsive to giving Fe nutrition so growth And development Lettuce Romaise (V3) more fast rather than varieties other lettuce.Hochmuth (2015) stated iron arrived around root as various compound chemistry or complex organic.Iron in solution land absorbed by the roots and excreted parts plant For replace missing elements due to the transpiration process or can used in the growth process.Roots can also obstruct Fe compounds inside land moment root grow and thrive to be the volume of soil addition.Density and extension root is very important factor in ability plant For obtain Fe.Fe absorption by plants is an active process, i.e energy released by plants For take Fe.Absorption iron depending on ability plant for 3+ reduces Fe 2+ to Fe in compound chelation.
Based on results analysis variety, show No There is difference the real one in interaction between varieties and nutrition to all variable observation increase tall plant lettuce at 15, and 45 days after (Table 1).As for the average tall plants that are affected by interactions varieties And nutrition on various age plant presented in Fig 1.

Wide Leaf
Results analysis variety to wide leaf show that treatment nutrition very significant differences in age plants 15 and 45 days after planting and treatment the varieties also differ very markedly in all age plants 15 and 45 HST, while in interaction nutrition and varieties different No real to all age plants (Table 1).
Results of DMRT test analysis (5%) in Table 4, Fe treatment Single EDDHA (N1) shows wide leaf widest, good at 15 days after birth or 45 hst, results the different real with Fe treatment Single EDTA (N2) and Compound Fe (N3).This is to be expected that single Fe EDDHA can sufficient Fe needs in plants, so formed leaves can grow with maximum, This thing in line with Opinion of David, et al,.2001 stated For fertilization substance iron, material chemistry best is EDDHA (Sequestration 138).Metal chelators it is very stable and able endure during a number of year inside land Because He recycle repeat many times for dissolve more Lots iron inside land and deliver it to root.Amount trace metal fertilizer used depending on the type material fertilizer as well as level severity shortcomings (David, et al., 2001).This is also reinforced by Hochmuth, et al., 2018) stated Iron is one of the of 16 elements important For growth and reproduction plants (some scientists also consider nickel is very important, making a total of 17).Iron (Fe) is one the most abundant element on the planet.In 1844, Eusebe Gris demonstrated that chlorosis certain plants can changed with nurse roots and leaves with solution iron.Substance iron is micronutrients and approx needed plant in amount small.Most of the plant annual requiring Fe 0.5 kg to 1.75 kg per hectare, comparably with nitrogen (N) ie around 40 kg to 100 kg per hectare.1991).Therefore that, plant with surface broad leaves will result necessary factors plant For photosynthesis will easy fulfilled so the process of photosynthesis will can walk with more maximum.Activity formation carbohydrate from the photosynthesis process will the more efficient so that can increase results (Rahayu, 2010).EDDHA fe-chelate does not only donate element iron but also donate nitrogen elements so influence protein and acid metabolism the amino.(Saprudin, 2010).Distance test analysis results Duncan's multiple (DMRT) on treatment varieties lettuce to wide leaf plant Lettuce that varieties Red lettuce (V2) shows influence wide leaf widest and results This different real with varieties lettuce other good at the age of 15 HST or 45 hst.
Table 5.Average wide leaf plant on treatment varieties aged 15 DAP and 45 DAP Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Results above show that Red Lettuce is more responsive to giving containing nutrients element Fe, as We know that The Fe element is a nutrient that plays a role in photosynthesis will produce food For used in development and growth plant like wide leaves and Fe also influence color leaf Because relate with content chlorophyll (Amalia and Rahayu, 2010).Plant level tall have two type chlorophyll that is chlorophyll a (C 55 H 72 O 5 N 4 Mg) which is colored green old and chlorophyll b (C 55 H 70 O 6 N 4 Mg) which is colored green young.Chlorophyll a and chlorophyll b are the strongest absorb light in parts red (600-700 nm), and the least absorb light green (500-600 nm).Whereas light colored blue absorbed by carotenoids.Carotenoids help absorb light, so spectrum light sun can utilized with more Good.Energy absorbed by chlorophyll b and carotenoids continued to chlorophyll a for used in the process of photosynthesis phase I (reaction bright) which consists from photosystems I and II, as well as chlorophyll -b.Chlorophyll a is the most abundant found in Photosystem II whereas Chlorophyll b is the most abundant found in Photosystem I (Nio, 2011).The more big wide leaf something plants, then expected content chlorophyll also increases lots and photosynthesis is possible taking place optimally.This matter accordingly with Gardner's (1991) opinion states that quantity and size leaf influenced by genotype and environment.Plant will do adaptation to change environment outside from optimum level and can finish stages phase growth origin circumstances environment No exceed limit physiological (Sitompul and Guritno, 1995)

Leaf Length
Analysis results variety to wide leaf show that treatment nutrition very significant differences in age plants 15 and 45 days after planting and treatment the varieties also differ very markedly in all age plants 15 and 45 DAP, while in the interaction nutrition and varieties different No real to all age plants (Table 1).
Results of DMRT test analysis (5%) in Table 6, EDDHA Single Fe treatment (N1) shows leaf length longest, good at age 15 DAT or 45 hst, results the different real with EDTA Single Fe (N2) and Compound Fe (N3) treatments.This is to be expected that single Fe EDDHA can sufficient Fe needs in plants, so formed leaves can grow with maximum.This matter in line with opinion Bozorgi, 2012, states that Iron (Fe) is essential nutrients Because become part from enzymes -enzymes particular and part of the proteins involved in the transfer of electrons in the phase bright photosynthesis and respiration

87
(Bozorgi, 2012).Leaf is an important organ plants play a role to photosynthesis.Leaf area (length leaves) and quantity high chlorophyll will causes the process of photosynthesis walk with Good.Growth amount leaf relate with activity photosynthesis, which produces food For need reserve food.The more long leaves, then the more wide surface leaf with thereby results photosynthesis tall so that plant grow with good (kusumah 2011).This is also reinforced by Hochmuth, et al., 2018) stated Iron is one of the of 16 elements important For growth and reproduction plants (some scientists also consider nickel is very important, making a total of 17).Iron (Fe) is one the most abundant element on the planet.In 1844, Eusebe Gris demonstrated that chlorosis certain plants can changed with nurse roots and leaves with solution iron.Substance iron is micronutrients and approx needed plant in amount small.Most of the plant annual requiring Fe 0.5 kg to 1.75 kg per hectare, comparably with nitrogen (N) ie around 40 kg to 100 kg per hectare.(Gardner et al, 1991).Therefore that, plant with surface broad leaves will result necessary factors plant For photosynthesis will easy fulfilled so the process of photosynthesis will can walk with more maximum.Activity formation carbohydrate from process photosynthesis will the more efficient so that can increase results (Rahayu, 2010).Fe-chelate EDDHA does not only donate element iron but also donate nitrogen elements so influence protein and acid metabolism the amino.(Saprudin, 2010).
Test analysis results distance Double Duncan (DMRT) on treatment varieties lettuce to long leaf plant Lettuce that varieties lettuce Romaeni (V3) shows influence wide leaf widest and results This different real with varieties lettuce other good at the age of 15 HST or 45 hst.Availability element hara is matter Which very important for growth And development plant, Because content element hara help expedite process metabolism plant among them process photosynthesis so that photosynthate produced high, the next one can translocated to all over part plant as a result will influential to growth long leaf (Yunus, 2013).According to Kloepper, 1993 (in Asngad, 2013), micro nutrients (Fe, Mn, Zn, B, Cu, and Mo) only needed plant in amount a little, however role important as catalyst during plant metabolic processes.In cultivation hydroponics, necessity nutrient No obtained from land, but rather obtained plant through irrigation at a time fertilization (fertigation), so internal nutrients fertilizer that must available and complete in fulfil need plant For growth And its development Based on results analysis variety, show No There is difference the real one in interaction between varieties and nutrition to all variable observation increase tall plant lettuce at 15, and 45 days after (Table 1).The average leaf length plants that are affected by interactions varieties And nutrition on various age plant presented in Figure 3.  Research result state that, there is interactions that don't real between treatment nutrition with treatment varieties to variable long leaf.This matter allegedly that there is difference response plant lettuce consequence treatment a number of varieties and nutrition provided.Growth beginning leaf because of the apical and mariginal meristems, which are both have In dicots layer The outermost marginal meristem divides anticlinal and not depending on the layer the cell below it.Expansion in surface associate with enhancement quantity and size chloroplast as well as amount Chlorophyll is found in palisade and sponges parenchyma.Arrangement cells network pallidase each other attached, but reach side long with chloroplast attached edge of the cold.This matter specialized For efficiency photosynthesis or dimensions area surface free beside That exists bones leaf Small plays a big role in spread current transpiration through mesophyll and plays a role as point beginning absorption results photosynthesis and translocation to outside leaf.Cell intermediate (cell between mesophyll and sieve elements) in bone minor leaves are appropriate with draft that cell transfer carbohydrate to deep conduit phloem need energy.For used in growth and storage (Lakitan, 2007).

Amount Leaf
Results analysis variety to amount leaf show treatment nutrition very significant differences in age plants 15 and 45 days after planting and treatment Varieties also differ very significantly in age 15 plants, medium at 45 days after planting show No different real, while in interaction nutrition and varieties different No real to all age plants (Table 1).
Results of DMRT test analysis (5%) in Table 8, Fe treatment Single EDDHA (N1) shows amount leaf most, both at the age of 15 DAT or 45 hst, results the different real with Fe treatment Single EDTA (N2) and Compound Fe (N3).This is to be expected that single Fe EDDHA can sufficient Fe needs in plants, so spur formation new leaves.Plant absorb iron in the form of ferric ion (Fe3+) in an aerobic 2+ atmosphere or ferrous (Fe) in an atmosphere anaerobic but generally there is in ferric form of phosphoprotein.Other forms are also absorbed plant is Fe(OH)2+ and Fe chelate.Generally makes up 0.01% of the plant with deep Fe range the leaves is 10-1000 ppm, but rate its sufficiency only 50-70 ppm.Plants that have content iron in amount enough, yes acidify rhizosphere, so cause release of Fe from compound binding (Hanafiah, 2012).Fertilizer iron grouped become three class main : soluble inorganic Fe compounds like iron (II) sulfate (FeSO4), Fe chelate synthetic such as ethylenediamine-N (EDDHA), ethylenediaminetetraacetic acid (EDTA) and natural Fe complexes (humates and amino acids).Lack substance iron reflect physiology and biochemistry from all over plants, because Fe is factor companion important from Lots enzymes, including those involved in biosynthetic from chlorophyll.Increase amount of Fe available for plant has been done for a long time through application Fe fertilizer.Cations For land and irrigation water, as well For plant seeds, roots, shoots and leaves (Abadıa, et al., 2011).Fe chelate fertilizer keep Fe in form dissolve and with thereby increase more Fe absorption lots of plants.With adding Fe chelate to plants can increase applied Fe translocation to another part of plants (Ylivainio, et al., 2004).Analysis results variety to heavy fresh root state that varieties lettuce red (V2) produces an average number leaf the most namely 12.93 p allegedly influenced by the nutrition provided and the genetics of the variety lettuce red (V2) ones capable produce leaf the most compared with varieties which other.Availability element hara is matter Which very important for growth dann development plant, Because content element hara help expedite process metabolism plant among them process photosynthesis so that photosynthate produced high, the next one can translocated to all over part plant as a result will influential to growth long leaf (Yunus, 2013).According to Kloepper, 1993(in Asngad, 2013), micro nutrients (Fe, Mn, Zn, B, Cu, and Mo) only needed plant in amount a little, however role important as catalyst during plant metabolic processes.In cultivation hydroponics, necessity nutrient No obtained from land, but rather obtained plant through irrigation at a time fertilization (fertigation), so internal nutrients fertilizer that must available and complete in fulfil need plant For growth And its development.

Heavy Fresh Root
Results distance test analysis Multiple Duncan ( where short roots and congested No absorb nutrition And water from land with efficient, p This can slow down growth lettuce.Different case with system plant use hydroponics, need nutrition And water plant can direct absorbed root.Function root For absorb nutrition Which later transported all over header plant so that expand system rooting For obtain supply hara more.Long root decrease with increase concentration nitrogen describe that use nitrogen more focused on growth header.Table 13.Average long leaf plant on treatment varieties aged 15 DAP and 45 DAP.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.As for interaction long root plant age 45 hst line First show that treatment nutrition Single Fe EDDHA in varieties lettuce k curly (N1V1) different No real with interaction Fe EDDHA nutrition in varieties lettuce red (N1V2) and interaction nutrition Single Fe EDDHA in varieties romaine lettuce (N1V3) with average long root plant 22 cm.Line second treatment interaction EDTA Single Fe nutrition in varieties lettuce curly (N2V1) is different No real with interaction EDTA Single Fe nutrition in varieties lettuce red (N2V2) and interaction nutrition Fe Single EDTA in varieties romaine lettuce (N2V3) with average long root plant 18 cm.Line third treatment interaction nutrition Fe Compound in varieties lettuce red (N3V2) is different real with interaction Compound Fe nutrition in varieties lettuce curly (N3V1) and interactions Compound Fe nutrition in varieties romaine lettuce (N3V3) with average 10 cm.   1).As for the results analysis descriptive to average fresh weight plant presented on Figure 3. From Figure 3, it can be seen variable fresh weight of the variety Lettuce red (V2) indicates heavy supreme fresh namely 69.70 grams is quoted with varieties romaine lettuce (V3) 60.49 grams and lettuce curly (V1) 47.96 grams (Figure 4).According to Ahmed (2010), biomass, diameter And tall plant influenced by varieties or genotype every plant.Every varieties own their respective advantages, good That from facet tall plant, diameter or biomass.As for the average root fresh weight which is influenced by interactions nutrition and varieties presented on /© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/)

Figure 1 :
Figure 1: Field Experiment Chart Data analysis methodThe analytical method used in this research uses ANOVA analysis of variance.If the results have a real effect then continue with the Duncan test (DMRT).The data analysis method for a 2-factor completely randomized design is as follows: Yijk = µ + αi + βj + γik + (αβ)ij + €ijk Information: Yijk = Observations on the kth experimental unit that received a combination of treatment at the ith level of factor N and the jth level of factor V, µ = True average value (population average), αi = Additive effect of the th level -i of factor N, βj= Additive influence of the jth level of factor V, (αβ)ij = Additive influence of the ith level of factor N and jth level of factor V, γik = Random effect of the main plot, which arises from the ith level of factor A in the kth replication, €ijk = Random effect of the kth experimental unit that received the treatment combination ij.Research ImplementationThis research began with preparation for the installation of the research site, namely making a hydroponic rack.The hydroponic rack uses 3" PVC pipe gavalum, the distance between holes is 16.2 cm, each rack has 36 planting holes, this research uses three racks as replications (figure1).The plant nursery begins by cutting the rockwool using a hacksaw with a small section size of 1.5 x 1.5 x 1.5 cm.Before sowing the cut rockwooll, water it until it is wet, then make a planting hole the size of a lettuce seed, then fill each hole in the rockwoll with 1 lettuce seed.Then observe the seeds every day.If there are any seeds that break/sprout, they can be moved to a place exposed to sunlight, keep the rock wool always wet and do this process until 4 lettuce leaves ready to be transplanted into the hydroponic installation that has been prepared.AB mix nutrients are in granular form consisting of stock A and stock B solutions, each weighing 125.There are 3 AB mix nutrients which have different micro Fe content, namely: single Fe EDDHA, Fe EDTA and compound micro Fe.Provide 2 bottles of 1 liter size, to make the dissolving process easier, also provide 2 buckets.then each bucket with 500 ml of raw water, put the stock A and stock B solutions into the bucket provided, stir until completely dissolved in the water.After that, put stock A and B in a 1 liter bottle and add more raw water until each becomes 1 liter.After that, enter stock A and stock B solutions, 45 ml each, into a nutrient water tank containing 15 liters.AB mix nutrition is given at the beginning of filling the nutrient water tank and if the nutrient water tank decreases by giving an AB mix concentration of 800 ppm.The observation variables observed include the following: 1. Length of leaves (cm), 2. Number of leaves (pieces).3. Leaf width (cm), 4. Plant height (cm), 5. Root length (cm), 6. Root fresh weight (g), 7. Plant fresh weight (g) /© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/) 84 Fe (N3) nutrients.This matter suspected in treatment nutrition Single Fe EDDHA (N1) capable produce amount chlorophyll more Lots compared with treatment other.This matter strengthened with opinion of Amaila and Rahayu (2010) where addition of Fe until with 6 ppm on plants capable increase tall plants, quantity leaves, and broad leaf matter This because Fe has role in formation chlorophyll so that the more lots of Fe available in plant so activity photosynthesis will the more increase.Results from photosynthesis This Then used as source food And energy For growth And development organ plant.Distance test results multiple Duncan (DMRT) treatment varieties show influence different very real to parameter tall plant on aged 15 and 45 HST.Average tall plant on treatment varieties presented on Table visible that Single Fe treatment EDDHA with varieties lettuce romaine (N1V3) shows tendency give results tall plant highest, p This allegedly giving from Fe EDDHA influence tall plant lettuce Because use fertilizer micro Fe Also will influence or donate element nitrogen.Long plant influenced by content nitrogen And phosphate in the solution formula nutrition provided.Nitrogen works For spur growth on phase vegetative especially leaf leaf And bars (Lakitan, 2007).

a
and chlorophyll b are the strongest absorb light in parts red (600-700 nm), And most A little absorb light green (500-600 nm).Whereas light colored blue absorbed by carotenoids.Carotenoids help absorb light, so spectrum light sun can utilized with more Good.Energy absorbed by chlorophyll b and carotenoids continued to chlorophyll a for used in the process of photosynthesis phase I (reaction bright) which consists from photosystems I and II, as well as chlorophyll -b.Chlorophyll a most Lots found in Photosystem II whereas Chlorophyll b is the most abundant there is on Photosystem I (Nio, 2011).

Figure 2 .
Figure 2. Average interaction nutrition and varieties on length leaves 15,30 and 45 HST.

Figure 2
Figure 2 states that at the age of 15 HST interaction EDDHA Single Fe nutrition with varieties romaine lettuce (N1V3) 8.22 cm states results highest and Compound Fe interactions with varieties lettuce curly (N3V1) 1.94 cm states results Lowest.At the age of 30 HST interaction EDDHA Single Fe nutrition with variety romaine lettuce (N1V3) 15.7 cm states results highest and interaction Compound Fe nutrition with varieties lettuce curly (N3V1) 3.6 cm states results Lowest.At the age of 45 hst interaction EDDHA Single Fe nutrition with varieties lettuce red (N1V2) 19.71 cm states results highest and Compound Fe interactions with varieties lettuce curly (N3V1) 4.68 states results lowest (Figure 2).Research result state that, there is interactions that don't real between treatment nutrition with treatment varieties to variable long leaf.This matter allegedly that there is difference response plant lettuce consequence treatment a number of varieties and nutrition provided.Growth beginning leaf because of the apical and mariginal meristems, which are both have /© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/) 89 pattern cleavage.

Table 8 .
Average amount leaf plants in treatment nutrition on ages 15 DAP and 30 DAP Information : Average Which followed by letters Which The same on column Which The same show different No real on test distance Double Duncan level 5% The number of leaves increases as the plant height increases will have an effect on the chlorophyll in the leaves also increasing, where chlorophyll in leaf role as absorption light For carry out photosynthesis (Siswandi and Sarwono, 2013).The number of leaves is related to stem growth or plant height where the stem is composed /© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/) 90 of segments stretching between the nodes of the stem where the leaves are located.Number of books and segments equal to the number of leaves.So as the length of the stem increases This will cause the number of leaves to form to increase.Growth tall plant happen consequence from elongation And increase segment on stem.Elongation segment happen Because exists activity cause increase in number cell.This process does not separated from internal physiological activities plant body (Ainina, 2018).A similar thing was expressed by Syahputra et al., (2014) Which state that tall plant related with amount leaf, because the leaves are located at the node of the plant stem, the greater the height of the plant And number of leaves, then fresh weight plants will increase.Test analysis results distance Double Duncan (DMRT) on treatment varieties lettuce to amount leaf plant Lettuce ona aged 15 years old show varieties lettuce lettuce curly (V1) shows influence amount leaf most and results This different with varieties lettuce Romaeni (V3), aged 45 DAP, showed influence No different real, however varieties lettuce Curly (V1) has trend amount leaf more Lots rather than varieties lettuce others (table9).
/© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/) 94 Solution pH level nutrition also determined by the plant absorb internal nutrients balance of anions and cations (Ratna, 2018).EDDHA is one of them chelate or wrapping form scratch organic so that the nutrients remain Can exchanged or absorbed by plants with Good.EDDHA's functions include: guard fixed nutrients Can absorbed plant when the pH of the solution soaring become too sour or base.element iron (Fe) if bonded with EDDHA becoming Fe-EDDHA, the nutrient Fe is still Can absorbed plant in pH range 3-10.Condition This different, if without exists EDDHA chelate.When the pH drops to number 4 for example, the Fe element No Can absorbed by plants.Meanwhile, Fe-EDTA still contains nutrients Can absorbed plant in pH range 2-6.5, Table 15.Average fresh weight of plant Which influenced by treatment nutrition.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Whereas influence varieties lettuce to variable fresh weight of the plant show different No real.(Table

Figure 3 .
Figure 3. Average heavy fresh plants Which influenced by varieties.From Figure3, it can be seen variable fresh weight of the variety Lettuce red (V2) indicates heavy supreme fresh namely 69.70 grams is quoted with varieties romaine lettuce (V3) 60.49 grams and lettuce curly (V1) 47.96 grams (Figure4).According to Ahmed (2010), biomass, diameter And tall plant influenced by varieties or genotype every plant.Every varieties own their respective advantages, good That from facet tall plant, diameter or biomass.As for the average root fresh weight which is influenced by interactions nutrition and varieties presented on Figure4.
Figure 3. Average heavy fresh plants Which influenced by varieties.From Figure3, it can be seen variable fresh weight of the variety Lettuce red (V2) indicates heavy supreme fresh namely 69.70 grams is quoted with varieties romaine lettuce (V3) 60.49 grams and lettuce curly (V1) 47.96 grams (Figure4).According to Ahmed (2010), biomass, diameter And tall plant influenced by varieties or genotype every plant.Every varieties own their respective advantages, good That from facet tall plant, diameter or biomass.As for the average root fresh weight which is influenced by interactions nutrition and varieties presented on Figure4.

Table 1 .
. Analysis results variant influence Nutrition, Variety Plants and Interactions between Nutrition with *: very significantly different, *: different real, ns: no different real.

Table 2 .
Average tall plants affected by treatment nutrition on aged 15, and 45 years old Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Results analysis test distance Multiple Duncan (DMRT) on parameter observation tall plants in treatment nutrition plant show that on age plant 15 hst and 45 hst different very real in all type nutrition.Single Fe Nutrient EDDHA (N1) yields avg the highest at each age plant And Then followed by Single Fe EDTA (N2) and Compound

Table 3 .
Average tall plant on treatment varieties at the age of 15 DAP and 45 DAP.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Test analysis results distance Double Duncan (DMRT) at high parameters affected plants by variety show that on aged 15 years old all implementation varieties lettuce different real, varieties Romaine lettuce shows tall plant highest namely 7.65 cm, whereas tall plants at 45 days after planting treatment varieties Romaine lettuce (V3) is tall plant highest (28.61 cm) results This different real with implementation other varieties.

2833-5376 Volume 03 Number 01 (January) 2024 Impact Factor: 9.51 SJIF (2023): 3.916 www.inter-publishing.com 2833
states that at the age of 15 HST interaction nutrition Single Fe EDDHA with varieties lettuce romaine (N1V3) 9.17 cm state results highest and Compound Fe interactions with varieties lettuce curly (N3V1) 2.78 cm stated results Lowest.At the age of 30 HST interaction nutrition Single Fe EDDHA with variety lettuce romaine (N1V3) 20.27 cm state results highest And interaction nutrition Fe Compound with varieties lettuce curly (N3V1) 4.78 cm states results Lowest.At the age of 45 hst interaction nutrition Fe Single EDDHA with varieties lettuce curly (N1V3) 42.51 cm states results highest and AB Mix micro interactions compound Fe with varieties lettuce curly (N3V1) 6.34 cm states results ISSN: -5376 /© 2024 The Authors.Published by Academic Journal INC.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/)

Table 4 .
Average wide leaf plants in treatment nutrition on ages 15 DAP and 30 DAP Information : Average Which followed by letters Which The same on column Which The same show different No real on test distance Double Duncan level 5% Leaf works as the main organ photosynthesis in plants level tall.Surface outside leaves are broad and flat possible ISSN:

Table 6 .
Average long leaf plants in treatment nutrition on ages 15 DAP and 30 DAP Information : Average Which followed by letters Which The same on column Which The same show different No real on test distance Double Duncan level 5% Leaf works as organ main photosynthesis on plant level tall.Surface outside leaves are broad and flat possible plant capable catch light maximum possible per unit volume and minimize required distance carried by CO 2 from surface leaf to chloroplasts

Table 7 .
Average long leaf plant on treatment varieties aged 15 DAP and 45 DAP.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Fe is hara Which role in photosynthesis Which will produce food for the above Results show that Romaine lettuce is more responsive to giving containing nutrients element Fe, as We know that Element used in development and growth plant like wide leaf And Fe join in influence color leaf Because relate with content chlorophyll (Amalia and Rahayu, 2010).Plant level tall have two type chlorophyll that is chlorophyll a (C 55 H 72 O 5 N 4 Mg) colored ones green old and chlorophyll b (C 55 H 70 O 6 N 4 Mg) colored ones green young.Chlorophyll ISSN:

Table 9 .
Average amount leaf plant on treatment varieties aged 15 DAP and 45 DAP.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Results above show that Lettuce Curly more responsive to giving containing nutrients Fe element, so Fe is absorbed by Lettuce curly more Lots used For formation leaf.Matter This allegedly each varieties lettuce give response Which different to component growth And results plant lettuce.There is difference This caused by genetic every varieties plant lettuce own characteristic different physique, shape, color and size.Variety plant the lettuce different show response different growth and yield although planted on the environment the same one as well as treatment the same nutrition, p This in accordance with Sadjad's opinion, 1993 in Marliah et al., 2012.

2833-5376 Volume 03 Number 01 (January) 2024 Impact Factor: 9.51 SJIF (2023): 3.916 www.inter-publishing.com 2833
table 10)variable observation fresh weight roots affected by treatment nutrition state very different in every treatment, Single Fe EDDHA (N1) 6.85 grams, Fe treatment EDTA Single (N2) 4.41 grams and treatment Compound Fe (N3) 1.2 grams (Table10).For get efficiency giving optimal nutrition, nutrition must done in sufficient quantity need plant.When plant given nutrition too Lots can vegetative And can cause poisoning for plant.On the contrary If given nutrition too A little can cause inhibition development root, so bother uptake nutrition plants, though plant the No show symptom deficiency visually (Sutedjo, 2010).Although iron only required plant For growth in small amount but if element iron No sufficient for plant so plant will show symptom initial abnormality with yellowing leaves young ones Then followed with death network (chlorosis)(Bennett, 1993).

Table 10 .
Average heavy fresh root Which influenced by treatment nutrition Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Test analysis results distance Double Duncan (DMRT) (table11) on treatment varieties lettuce to fresh weight of Lettuce show that varieties lettuce Romaeni (V3) shows influence heaviest to fresh weight of the plant results This different real with varieties lettuce curly (V1), fixed No different real with varieties red (V2).This matter allegedly every varieties lettuce own characteristics different in the root.Root is a vegetative organ main supply of water, minerals and materials the important one For growth and development plant.System rooting plant more controlled by nature genetic from the plant concerned, condition land or planting medium.On the contrary absorption nutrients and water by root very determine growth plant(Manuhutu, et al, 2014).

Table 11 .
Average long leaf plant on treatment varieties aged 15 DAP and 45 DAP.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.

Table 12 )
. It's expected that Single Fe EDDHA is capable trigger happen division cells in roots plant Lettuce, so formation cells new one can extend roots in plants lettuce, p This in line with opinion Handayanim, et al., 2007 stated fertilization Fe done with apply fertilizer micro Which contain Fe-EDDHA or Fe-EDTA, can control several metabolic processes diankar among them absorption micro nutrition by cells -cell roots, movement micro nutrition from root to shoots, and abilities network leaf For fill in element nutrition the to vessels phloem.More Amalia, et al., (2010) continued to explain that plant absorb nutrition from the planting medium through root, with more roots long and more big, so will expand surface uptake nutrition plant so that plant obtain nutrition ISSN:

Table 12 .
Average long root Which influenced by treatment nutrition.Information : Average Which followed letter Which The same on column Which The same show different No real on test DMRT level 5%.Test analysis results distance Double Duncan (DMRT) (table 13) on treatment varieties lettuce to long root plant Lettuce show that varieties Red lettuce (V2) shows long root longest results This different real with varieties lettuce curly (V1), but No different real with Romaine variety (V3).This is to be expected root varieties Red selana (V2) more responsive to treatment giving Fe nutrition, p This in line with opinion Hananin, et al., 2020

Table 14 .
Influence interaction nutrition And varieties on parameters observation long root 45 hst